PCR digital a Gotas
Digital PCR system drops QX200 (ddPCR ™) provides absolute measurement using DNA or RNA probes or EvaGreen. The QX200 system has several advantages over traditional media. The solution is more accurate and sensitive digital PCR for a variety of applications. It is optimized for TaqMan ™ probe hydrolysis and testing EvaGreen. It is simple and easy to use with a workflow up 96 samples. In partition 20,000 nano drops of sample bias reduces the amplification efficiency and the effect of PCR inhibitors. Additionally, this technique does not require calibration curves.
Applications of digital PCR system drops:
- Studies biomarkers cancer and copy number variation:
Measurement of various degrees of cancer mutations, detection of DNA copy number with very low sensitivity and high resolution.
- Pathogen Detection
Use QX200 extreme accuracy to quantify small changes in DNA or RNA target molecules used in the detection and monitoring of pathogenic.
- Next-generation sequencing (NGS)
Performs a precise quantification and qualification NGS libraries without the use of a standard curve.
- Gene expression
Achieve reliable and reproducible measurements of small changes in the abundance of mRNA and miRNA expression low.
- Food analysis
Allows routine assessment of genetically modified organisms (GMO) by validated methods for ddPCR